Tools:Standardized Analytical Methods
From TraceFood
In the following, table information is given about methods of analysis in a standardised manner. The format of method of analysis and explanation of the headings are as follows:
| Explanation of items in standardized analytical methods | |
| Method number | Number of all methods recorded within the ad-hoc standard |
| Method of analysis | Method name may include analyte and type of method |
| Category of method of analysis | Reference to official status of cooperating organisation |
| Parameter group | Main group of parameters like microbiological, chemical or others |
| Parameter | Analyte level |
| Legislative limit | Residual concentration allowed in e.g. EU directive of regulation |
| Monitoring | Subject to monitoring according to legislation |
| Literature reference | EU directive or published research reference of the method |
| Chemical Abstract Service | Registry number, a unique identifier to be used to search a number of data-retrieval systems |
| Cross reference | Method may also include references in other chapters/products |
Various additions to this standardized table of analytical methods are possible, but this depends on the level of thoroughness of data required. The following items may be considered to include in the table:
| Applicability statement: | Limitations on use of method or other information |
| Method performance: | Statistical information generated by collaborative study like, trueness %, precision % or limit of detection % |
| Principle: | Scientific basis for the method of analysis |
| Apparatus and reagents: | Specifications for necessary laboratory apparatus and reagent preparations |
| Preparation of sample: | Description of method of analysis in several sections |
| Calculation: | Description of final result of method of analysis |
Method number 1
| Method of analysis | HPLC, GC |
| Category of method of analysis | |
| Parameter group | Carbohydrates |
| Parameter | a. Fructose and glucose
b. Sucrose |
| Legislative value | a. not less than 60g/100g
b. Not more than 5 g/100g (in general) |
| Monitoring | |
| Literature reference | EU directive 2001/101/EC |
| Chemical Abstract Service | a. 57-48-7 fructose
b. 50-99-7 glucose c. 57-50-1 sucrose |
| Cross Reference |
Method number 2
| Method of analysis | Filter method |
| Category of method of analysis | |
| Parameter group | Insoluble matter |
| Parameter | Water insoluble solids contents |
| Legislative value | Not more than 0,1g/100g (in general)
Not more than 0,5g/100g (pressed honey) |
| Monitoring | To detect honey impurities |
| Literature reference | EU directive 2001/101/EC
Chemical Abstract Service |
| Cross reference |
Method number 3
| Method of analysis | Isotope ratio mass spectrometry (13C/12C ratio) |
| Category of method of analysis | |
| Parameter group | |
| Parameter | Cane and maize syrups |
| Legislative value | |
| Monitoring | To detect any form of adulteration |
| Literature reference | AOAC
Chemical Abstract Service |
| Cross reference |
Method number 4
| Method of analysis | Microscopy |
| Category of method of analysis | |
| Parameter group | Botanical |
| Parameter | Pollen |
| Legislative value | |
| Monitoring | |
| Literature reference | Moar, N.T. (1985) Pollen analysis of New Zealand honey. New Zealand Journal of Agricultural Research. 1985. vol. 28, 39-70
Chemical Abstract Service |
| Cross reference |
Method number 5
| Method of analysis | HPLC LD or UV, Winkler, Bisulfite White |
| Category of method of analysis | |
| Parameter group | Chemical |
| Parameter | Hydroxy Methyl furualdehyde (HMF) |
| Legislative value | a. not more than 40 mg/kg (in general, except baker’s honey) (EU)
b. not more than 80 mg/kg (honeys of declared origin from regions with tropical climate and blends of these honeys) (Codex) |
| Monitoring | |
| Literature reference | a. EU directive 2001/101/EC
b. Codex Stan 12-1981 |
| Chemical Abstract Service | 67-47-0 |
| Cross reference |
Method number 6
| Method of analysis | Refractometry (Carl Fisher method) |
| Category of method of analysis | |
| Parameter group | |
| Parameter | Moisture |
| Legislative value | a. Not more than 20% (in general)
b. Not more than 23% (heather and baker’s honey in general) c. Not more than 25% (baker’s honey from heather) |
| Monitoring | Quality criteria determines capability of honey to remain stable |
| Literature reference | c. EU directive 2001/101/EC |
| Chemical Abstract Service | |
| Cross reference |
Method number 7
| Method of analysis | Spectrophotometry
VIS photometer, Lovibond comparator or Lovibond color Pod, Hanne color analyser |
| Category of method of analysis | |
| Parameter group | physical |
| Parameter | Colour |
| Legislative value | |
| Monitoring | |
| Literature reference | |
| Chemical Abstract Service | |
| Cross reference |
Method number 8
| Method of analysis | Viscosity |
| Category of method of analysis | |
| Parameter group | Organoleptical |
| Parameter | Texture |
| Legislative value | |
| Monitoring | |
| Literature reference | Mendes, E. , et al. (1998). Quality evaluation of Portugese honey. Carbohydrate Polymers. Vol. 37, issue 3, nov. 1998, 219-223 |
| Chemical Abstract Service | |
| Cross reference |
Method number 9
| Method of analysis | |
| Category of method of analysis | |
| Parameter group | organoleptical |
| Parameter | smell |
| Legislative value | |
| Monitoring | |
| Literature reference | Mendes, E. , et al. (1998). Quality evaluation of Portugese honey. Carbohydrate Polymers. Vol. 37, issue 3, nov. 1998, 219-223 |
| Chemical Abstract Service | |
| Cross reference |
Method number 10
| Method of analysis | |
| Category of method of analysis | |
| Parameter group | Organoleptical |
| Parameter | Taste |
| Legislative value | |
| Monitoring | |
| Literature reference | Mendes, E. , et al. (1998). Quality evaluation of Portugese honey. Carbohydrate Polymers. Vol. 37, issue 3, nov. 1998, 219-223 |
| Chemical Abstract Service | |
| Cross reference |
Method number 11
| Method of analysis | Spectrophotometric measurement of 4-nitrophenol |
| Category of method of analysis | |
| Parameter group | |
| Parameter | Enzyme activity (invertase) |
| Legislative value | |
| Monitoring | Freshness indicator |
| Literature reference | SiegenthalerU., 1977. Eine einfache und rasche Methode zur Bestimmung der alpha-Glucosidase (Saccharase) im Homig, Mitt. Gebiete Lebensm. Hyg. 68, pp. 251–258. |
| Chemical Abstract Service | |
| Cross Reference |
Method number 12
| Method of analysis | Schade or Phadebas method |
| Category of method of analysis | |
| Parameter group | |
| Parameter | Diastase activity |
| Legislative value | a. Not less than 8 Schade units( in general, except baker’s honey)
b. Not less than 3 Schade units (honeys with low natural enzyme content and HMF content of not more than 15 mg/kg) |
| Monitoring | |
| Literature reference | EU directive 2001/101/EC |
| Chemical Abstract Service | |
| Cross reference |
Method number 13
| Method of analysis | Conductimeter |
| Category of method of analysis | |
| Parameter group | |
| Parameter | Electrical conductivity |
| Legislative value | a. not more than 0,8 mS/cm (honey in general, except see legislation)
b. not less than 0,8 mS/cm (honeydew and chestnut honey, except see legislation) |
| Monitoring | Depends on ash and acid content |
| Literature reference | EU directive 2001/101/EC |
| Chemical Abstract Service | |
| Cross reference |
Method number 14
| Method of analysis | Liquid and Gas Chromatography (multi analysis), HPLC |
| Category of method of analysis | |
| Parameter group | Chemical (acaricides) |
| Parameter | a. Flumethrine (prohibited for bees)
b. Tau-Fluvalinate (prohibited for bees) c. Coumaphos |
| Legislative value | a. zero
b. zero c. 0.1 mg/kg |
| Monitoring | No EU legislation (possibility Directive 96/23/EC) |
| Literature reference | a. Regulation 2686/98/EC
b. Regulation 2034/96/EC c. Regulation 1931/99/EC Martel AC, Zeggane S. Determination of acaracides in honey by high-performance liquid chromatography with photodiode array detection. J. Chromatogr.A 2002 Apr 19; 954 (1-2):173-80 Sanchez-Brunette C, Albereo B, Miguel E, Tadeo JL. Determination of insecticides in honey by matrix solid-phase dispersion and gaschromatography with nitrogen-phosphorus detection and mass spectrometric conformation. J. AOAC 2002 Jan-Feb; 85 (1):128-33 Korta E, Bakkali A, Berruetta LA, Gallio B, Vincente F. Study of an accelerated solvent extraction procedure for the determination of acaracide residues in honey by high-performance liquid chromatography-diode array detector. J.Food Prot. 2002 Jan;65(1):161-6 Menkissoglu-Spiroudi U; Diamantidis GC, Georgiou VE, Thrasyvoulou AT, Determination of malathion, coumafos and fluvalinate residues in honey by gas chromatography with nitrogen-posphorus or electron capture detectors. J.AOAC INT. 200 Jan-Feb; 83 (1): 178-82 |
| Chemical Abstract Service | a. 69770-45-2
b. 102851-06-9 c. 56-72-4 |
| Cross reference |
Method number 15
| Method of analysis | LC /GC |
| Category of method of analysis | |
| Parameter group | Acaracides |
| Parameter | Oxalic acid |
| Legislative value | Not yet established (see also EU regulation 2377/90 annex II) |
| Monitoring | |
| Literature reference | Regulation 546/2004/EC
Moosbeckhofer, R. et al. 2003. Investigations on the oxalic acid content of honey from oxalic aced treated and untreated bee colonies |
| Chemical Abstract Service | 144-62-7 |
| Cross reference |
Method number 16
| Method of analysis | Gas chromatography |
| Category of method of analysis | |
| Parameter group | Antibiotics (Formamidines) |
| Parameter | Amitraze |
| Legislative value | 0.2 mg/kg |
| Monitoring | No EU legislation on monitoring (possibility Directive 96/23/EC) |
| Literature reference | Regulation 3093/92; 1430/94; 1742/96; 1916/98; 1863/97; 2395/99; 1646/2004
Jimenez JJ, Nozal MJ, Bernakl JL, Santos M, Mayorga AL. Factors affecting the extraction, hydrolysis and derivation steps for the quantitation of total residues of amitraz in honey by gas chromatography with electron capture detection. Anal Bioanal Chem 2002 Sep; 37 (2):300-4. Epub 2002 Aug 27 |
| Chemical Abstract Service | 33089-61-1 |
| Cross reference |
Method number 17
| Method of analysis | Liquid chromatography |
| Category of method of analysis | |
| Parameter group | Anitbiotics (Iminopphenyl thiazlidine derivatives) |
| Parameter | Cymiazole |
| Legislative value | 1 mg/kg |
| Monitoring | No EU legislation on monitoring (possibility Directive 96/23/EC ) |
| Literature reference | Regulation 2071/96; 1931/1999/EC
Cabras P, Melis M, Spanedda L. Determination ofcymiazole residues in honey by liquid chromatography. J.AOAC Int. 1993 Jan-Feb; 76 (1):92-4 |
| Chemical Abstract Service | 61676-87-7 |
| Cross reference |
Method number 18
| Method of analysis | Liquid chromatography and mass spectrometry |
| Category of method of analysis | |
| Parameter group | Antibiotic substance (prohibited for bees) |
| Parameter | Tylosin |
| Legislative value | zero |
| Monitoring | No EU legislation on monitoring (possibility Directive 96/23/EC ) |
| Literature reference | Regulation 1931/99
Nozal Nalda MJ, Bernal Yague JL, Gomez MT, Jimenez Sevilla JJ, Bernal del Nozal J, Higes Pascual M. Trace analysis of antibacterial tylosin A, B, C and D in honey by liquid chromatography-electrospray ionization-mass spectrometry. J Sep Sci. 2006 Feb;29(3):405-13 |
| Chemical Abstract Service | 1401-69-0 |
| Cross reference |
Method number 19
| Method of analysis | Liquid chromatography |
| Category of method of analysis | |
| Parameter group | Prohibited Antibiotics for bees (in EU) |
| Parameter | Streptomycin |
| Legislative value | Zero |
| Monitoring | No EU legislation on monitoring (possibility Directive 96/23/EC ) |
| Literature reference | 1140/96; 1530/2002
Edder, P. et al. 1999. Determination of streptomycin residues in food by solid phase extraction and liquid chromatography with post column derivatization and fluorometric detection. Journal of Chromatography. Vol. 830, 2, 345-351 |
| Chemical Abstract Service | 57-92-1 |
| Cross reference |
Method number 20
| Method of analysis | LC/MS/MS |
| Category of method of analysis | |
| Parameter group | Prohibited antibiotic |
| Parameter | Chloramphenicol |
| Legislative value | Zero |
| Monitoring | No EU legislation on monitoring (possibility Directive 96/23/EC ) |
| Literature reference | Regulation 675/92; 1430/94
Ortelli, D. 2004. Analysis Chloramphenicol residues in honey by liquid chromatography tandem mass spectrometry. Chromatographia. 2004, 59, 61-64 |
| Chemical Abstract Service | 56-75-7 |
| Cross reference |
Method number 21
| Method of analysis | LC/MS/MS |
| Category of method of analysis | |
| Parameter group | Prohibited antibiotics for bees |
| Parameter | Tetracycline |
| Legislative value | zero |
| Monitoring | No EU legislation on monitoring (possibility Directive 96/23/EC ) |
| Literature reference | Regulation 3462/93; 281/96
Ishii, R. et al. 2006. Analysis of tetracyclines in honey and royal jelly by LC/MS/MS. Shokuhin Eiseigaku Zasshi. 2006. dec, 47(6): 277-83 (Japanese article) |
| Chemical Abstract Service | 60-54-8 |
| Cross reference |
Method number 22
| Method of analysis | LC/MS/MS |
| Category of method of analysis | |
| Parameter group | Prohibited antibiotic for bees |
| Parameter | Sulfonamides (sulfanilamide) |
| Legislative value | zero |
| Monitoring | No EU legislation on monitoring (possibility Directive 96/23/EC ) |
| Literature reference | Regulation 675/92; 3426/93; 281/96
Huq, S. , K. Kallury. 2006. Extraction and analysis of sulfonamides from honey by LC/MS/MS using straat X-C polymeric SPE sorbent en Gemini C18 HPLC column. Appilcations SPE TN-012 |
| Chemical Abstract Service | 63-74-1 |
| Cross reference |
Method number 23
| Method of analysis | LC/MS/MS |
| Category of method of analysis | |
| Parameter group | Prohibited antibiotic |
| Parameter | Nitrofurane (nitrofurazone) |
| Legislative value | zero |
| Monitoring | No EU legislation on monitoring (possibility Directive 96/23/EC ) |
| Literature reference | Regulation 2901/93
Khong, SP. et el. 2004. Analysis of matris bound nitrofuran residues in worldwide originated honeys by isotooe dilution LC/MS/MS. J. Agric. Food chem.. 52 (17) 5309-5315. |
| Chemical Abstract Service | 59-87-0 |
| Cross reference |
Method number C1
| Method of analysis | Chemical test or (oven method) |
| Category of method of analysis | ISO 1442:1997 (Determination of moisture content in meat and meat products) |
| Parameter group | Composition |
| Parameter | Water content |
| Legislative value | Mean physiological W/RP ratio 3,19 (chicken breast fillet)
(W – water, RP = protein) |
| Monitoring | |
| Literature reference | EU regulation 1538/91 (annex V!a) |
| Chemical Abstract Service | 7732-18-5 |
| Cross Reference |
Method number C2
| Method of analysis | Chemical test or (Kjeldahl method) |
| Category of method of analysis | ISO 937:1998 (Determination of nitrogen content in meat and meat products) |
| Parameter group | Composition |
| Parameter | Protein content |
| Legislative value | Mean physiological W/RP ratio 3,19 (chicken breast fillet)
(W – water, RP = protein) |
| Monitoring | |
| Literature reference | EU regulation 1538/91 (annex V!a) |
| Chemical Abstract Service | |
| Cross reference |
Method number C3
| Method of analysis | Extract with ether |
| Category of method of analysis | ISO 1443-1973 |
| Parameter group | Composition |
| Parameter | Fat |
| Legislative value | Not applicable |
| Monitoring | |
| Literature reference | |
| Chemical Abstract Service | Fat16: 93376-67-1 |
| Cross reference |
Method number C4
| Method of analysis | Atomic Absorption Spectrometry (AAS)
Ashing triplicate (total iron) |
| Category of method of analysis | |
| Parameter group | Composition |
| Parameter | Mineral (Fe) |
| Legislative value | |
| Monitoring | |
| Literature reference | Clark, E.M. et al. 1997. Heme and total iron in ready-to-eat chicken. Journal Agric. Food Chemistry. 1997, 45, 124-126 |
| Chemical Abstract Service | 15438-31-0 (Fe 2+)
7439-89-6 (iron) |
| Cross reference |
Method number C5
| Method of analysis | Texture analyzer (Warner-Bratzler shear apparatus) |
| Category of method of analysis | |
| Parameter group | Organoleptical |
| Parameter | Texture |
| Legislative value | |
| Monitoring | |
| Literature reference | Wattanachant, S. 2004. Composition, Color, and Texture of Thai Indigenous and Broiler Chicken Muscles. Poultry Science 83: 123-128 |
| Chemical Abstract Service | |
| Cross reference |
Method number C6
| Method of analysis | Isolation of hemagglutinating virus |
| Category of method of analysis | |
| Parameter group | Diseases |
| Parameter | Newcastle Disease (ND) |
| Legislative value | |
| Monitoring | |
| Literature reference | The Merck Veterinary Manual |
| Chemical Abstract Service | |
| Cross reference |
Method number C7
| Method of analysis | Microscopically diagnosis of oocysts |
| Category of method of analysis | |
| Parameter group | Diseases |
| Parameter | Coccidiosis |
| Legislative value | Anticoccidials MRL 100µg/kg (EUReg. 205/2004) |
| Monitoring | |
| Literature reference | The Merck Veterinary Manual |
| Chemical Abstract Service | |
| Cross reference |
Method number C8
| Method of analysis | Clinical (pulmonair hypertensis syndrome) Oxygen deficiency, fast heart beat |
| Category of method of analysis | |
| Parameter group | Diseases |
| Parameter | Broilers Ascites Syndrome (BAS or water belly) |
| Legislative value | |
| Monitoring | |
| Literature reference | Ontario Ministry of Agriculture, Food and Rural Affairs |
| Chemical Abstract Service | |
| Cross reference |
Method number C9
| Method of analysis | Isolation of virus from tracheal and cloacal swabs |
| Category of method of analysis | |
| Parameter group | Diseases |
| Parameter | Avian Influenza (AI) |
| Legislative value | |
| Monitoring | |
| Literature reference | The Merck Veterinary Manual |
| Chemical Abstract Service | |
| Cross reference |
Method number C10
| Method of analysis | 1. Agar pour plate method
2. Spiral plating method 3. Isogrid system 4. Petriflim system 5. Redigel system |
| Category of method of analysis | ISO 10272-1 (2006) for Campylobacter
ISO 6579 (2002) for Salmonella |
| Parameter group | Microbiological |
| Parameter | Total bacterial count |
| Legislative value | See Salmonella (method C11) |
| Monitoring | EU monitoring system |
| Literature reference | EU directive 2003/99 (concerns monitoring zoonoses and zoonotic agents) |
| Chemical Abstract Service | |
| Cross reference |
Method number C11
| Method of analysis | Agar / chromogenic agars / immunogenic assay / PCR |
| Category of method of analysis | EN/ISO 6579 |
| Parameter group | Zoonoses |
| Parameter | Salmonella |
| Legislative value | From 1-1-2006 absence in 10 gr. Poultry meat intended to be eaten cooked
From 1-1-2010 absence in 25 gr. Poultry meat intended to be eaten cooked |
| Monitoring | EU monitoring system |
| Literature reference | EU directive 2003/99 (concerns monitoring zoonoses and zoonotic agents)
EU regulation 2160/2003 (concerns control of Salmonella and other specified food-borne zoonotic agents) EU regulation 2073/2005 (concerns microbiological criteria for foodstuffs) |
| Chemical Abstract Service | |
| Cross reference |
Method number C12
| Method of analysis | Agar / chromogenic agars / immunogenic assay / PCR |
| Category of method of analysis | |
| Parameter group | Zoonoses |
| Parameter | Campylobacter |
| Legislative value | |
| Monitoring | EU monitoring system |
| Literature reference | EU directive 2003/99 (concerns monitoring zoonoses and zoonotic agents) |
| Chemical Abstract Service | |
| Cross reference |
Method number C13
| Method of analysis | ASTED-HPLC-UV or HPLC-DAD |
| Category of method of analysis | |
| Parameter group | Antibacterial substances |
| Parameter | Sulphonamides (sulphonamid) |
| Legislative value | 100 µg/kg |
| Monitoring | EU national plan |
| Literature reference | EU Regulation 508/1999 |
| Chemical Abstract Service | 63-74-1 |
| Cross Reference |
Method number C14
| Method of analysis | LC-MS/HPLC |
| Category of method of analysis | |
| Parameter group | Antibacterial substances |
| Parameter | a. Oxytetracyclin
b. Tetracyclin c. Chlortetracylin d. Doxyclyclin e. Flumequine f. Enrofloxacin |
| Legislative value | a. 100 µg/kg
b. 100 µg/kg c. 100 µg/kg d. 100 µg/kg e. 400 µg/kg f. 100 µg/kg |
| Monitoring | EU national plan |
| Literature reference | a./b./c./d. EU regulation 508/1999
e./f. EU regulation 1121/2002 |
| Chemical Abstract Service | a. 79-57-2
b. 60-54-8 c. 57-62-5 d. 564-25-0 e. 42835-25-6 f. 93106-60-6 |
| Cross Reference |
Method number C15
| Method of analysis | HPLC-UV / HPLC-DAD |
| Category of method of analysis | |
| Parameter group | Anticoccidials |
| Parameter | a. Nicarbazin
b. Metichlorpindol |
| Legislative value | a. 100 µg/kg
b. 100 µg/kg |
| Monitoring | EU national plan |
| Literature reference | a. en b. EU directive 96/23 EC |
| Chemical Abstract Service | a. 330-95-0
b. 2971-90-6 |
| Cross Reference |
Method number C16
| Method of analysis | HPLC-UV / HPLC-LCMS |
| Category of method of analysis | |
| Parameter group | Anticoccidials |
| Parameter | Toltrazuril |
| Legislative value | 100 µg/kg |
| Monitoring | EU national plan |
| Literature reference | EU regulation 508/1999 and 206/2006 |
| Chemical Abstract Service | 69004-03-1 |
| Cross Reference |
Method number C17
| Method of analysis | HPLC-FD |
| Category of method of analysis | |
| Parameter group | Insecticides - Carbamates |
| Parameter | a. Aldicarb
b. Carbaryl c. Ethiofencarb d. Methomyl e. Propoxur |
| Legislative value | a. 10 µg/kg
b. 500 c. 20 d. 20 e. 50 |
| Monitoring | EU national plan |
| Literature reference | EU regulation 149/2008 |
| Chemical Abstract Service | a. 116-06-3
b. 63-25-2 c. 29973-13-5 d. 16752-77-5 e. 114-26-1 |
| Cross Reference |
Method number C18
| Method of analysis | GC-MS |
| Category of method of analysis | |
| Parameter group | Insecticides - Pyrethroids |
| Parameter | a. Cyhalothrin
b. Cyfluthrin c. Cypermethrin d. Deltamethrin e. Fenvalerate f. Flucythrinate g. Permethrin |
| Legislative value | a. 20 µg/kg
b. 50 µg/kg c. 50 µg/kg d. 50 µg/kg e. 10 µg/kg f. 20 µg/kg g. 500 µg/kg |
| Monitoring | EU national plan |
| Literature reference | EU regulation 149/2008 |
| Chemical Abstract Service | a. 68085-85-8
b. 68359-37-5 c. 52315-07-8 d. 52918-63-5 e. 51630-58-1 f. 70124-77-5 g. 52645-53-1 |
| Cross Reference |
Method number C19
| Method of analysis | HPLC |
| Category of method of analysis | |
| Parameter group | Non steroidal anti-inflammatory drugs |
| Parameter | Na-salicylate |
| Legislative value | 20 µg/kg |
| Monitoring | EU national plan |
| Literature reference | EU regulation 2377/1990 |
| Chemical Abstract Service | 54-21-7 (sodium salicylate) |
| Cross Reference |
Method number C20
| Method of analysis | LC-UV |
| Category of method of analysis | |
| Parameter group | Feed Additives |
| Parameter | a. Lasalocid
b. Narasin c. Salinomycin d. Monensin-sodium |
| Legislative value | a. 20 µg/kg
b. 50 µg/kg c. 5 µg/kg d. 20 µg/kg |
| Monitoring | |
| Literature reference | a. EU regulation 712/2005; 1055/2006; 1353/2006
b. EU regulation 545/2006 c. EU regulation 496/2007; 500/2007 d. EU regulation 108/2007; 109/2007 |
| Chemical Abstract Service | a. 25999-31-9
b. 55134-13-9 c. 53003-10-4 d. 22373-78-0 |
| Cross Reference |
Method number C21
| Method of analysis | LC-MS |
| Category of method of analysis | |
| Parameter group | Banned veterinary drugs |
| Parameter | a. Chloramphenicol
b. Nitrofurans (AMOZ, AOZ, AHD, SEM) c. Nitroimidazoles: Ronidazol, Dimetridazol, Metronidazol) d. Dapsone |
| Legislative value | a. 0,3 µg/kg (level of action)
b. 1 µg/kg (level of action) c. ? d. ? |
| Monitoring | EU national plan third countries |
| Literature reference | EU Directive 2377/1990 annex IV |
| Chemical Abstract Service | a. 25999-31-9
b. 67-45-8 c. 7681-76-7 (Ronidazole); 551-92-8 (Dimetridazol); 443-48-1 (Metronidazol) d. 80-08-0 |
| Cross Reference |
Method number C22
| Method of analysis | GC-ECD / GC-MS |
| Category of method of analysis | |
| Parameter group | Chlorinated hydrocarbons |
| Parameter | a. DDT
b. Chlordane c. Dieldrin/aldrin d. Endrin |
| Legislative value | a. 1000 µg/kg
b. c. 50 / 50 µg/kg d. 200 µg/kg |
| Monitoring | EU national plan |
| Literature reference | EU regulation 149/2008 |
| Chemical Abstract Service | a. 50-29-3
b. 57-74-9 c. 60-57-1 / 309-00-2 d. 72-20-8 |
| Cross reference |
Method number C23
| Method of analysis | GC-ECD / GC-MS |
| Category of method of analysis | |
| Parameter group | Organochlorine compounds |
| Parameter | a. HCB (ISO)
b. alpha-HCH c. beta-HCH d. gamma-HCH (Lindane) e. Heptachlor f. Methoxychlor |
| Legislative value | a. 50 µg/kg
b. 200 µg/kg c. 200 µg/kg d. 100 µg/kg e. 700 µg/kg f. 200 µg/kg |
| Monitoring | EU national plan |
| Literature reference | EU Directive 96/23/EC
EU Directive 2006/77 |
| Chemical Abstract Service | a. 118-74-1
b. 319-84-6 c. 319-85-7 d. 58-89-9 e. 1024-57-3 f. 72-43-5 |
| Cross reference |
Method number C24
| Method of analysis | GC-ECD / GC-MS |
| Category of method of analysis | |
| Parameter group | PCBs |
| Parameter | a. PCB 138
b. PCB 153 c. PCB 28 d. PCB 52 e. PCB 101 f. PCB 118 g. PCB 180 |
| Legislative value | a. 50 µg/kg
b. 50 µg/kg c. 50 µg/kg d. 20 µg/kg e. 40 µg/kg f. 40 µg/kg g. 60 µg/kg |
| Monitoring | EU national plan |
| Literature reference | EU regulation 199/2006
EU directive 2004/44/EC |
| Chemical Abstract Service | Not available |
| Cross reference |
Method number C25
| Method of analysis | Isotope ratio analysis |
| Category of method of analysis | |
| Parameter group | Stable isotopes |
| Parameter | a. 2H/1H,
b. 13C/12C c. 15N/14N d. 34S/32S e. 87Sr/86Sr |
| Legislative value | |
| Monitoring | Not applicable |
| Literature reference | Camin, F. et al. 2007. Multi element (H, C, N, S) stable isotope characteristics of lamb meat from different European regions. Analytical and Bio analytical Chemistry, vol 389, no. 1, 309-320. |
| Chemical Abstract Service | Not available |
| Cross reference |
Method number MW1
| Method of analysis | Total Colony count (per ml of water) by inoculation in a nutrient agar medium |
| Category of method of analysis | ISO 9308-1, ISO 7899-2, EN-ISO 12780, EN-ISO 6222 |
| Parameter group | Microbiological |
| Parameter | a. Escherichia coli and coliform bacteria
b. Enterococci c. Sporulated sulphite-reducing anaerobes d. Pseudomonas aeruginosa |
| Legislative value | a. 0/250 ml at 22oC and 37oC (absence)
b. 0/250 ml (absence) c. 0/50 ml (absence) d. 0/250 ml (absence) |
| Monitoring | Yes |
| Literature reference | EU directive 80/777/EEC |
| Chemical Abstract Service | |
| Cross Reference |
Method number MW2
| Method of analysis | Reverse transcription Polymerase Chain Reaction (RT-PCR) |
| Category of method of analysis | |
| Parameter group | Microbiological |
| Parameter | Norovirus |
| Legislative value | |
| Monitoring | |
| Literature reference | Applied and Environmental Microbiology. 2003, 69 (11) 6541 - 6549 |
| Chemical Abstract Service | |
| Cross reference |
Method number MW3
| Method of analysis | Gas chromatography (GC) incl. solvent extraction |
| Category of method of analysis | |
| Parameter group | Chemical |
| Parameter | Bromate |
| Legislative value | 10 μg/l (EU); 25 μg/l (guideline WHO, 1998) |
| Monitoring | |
| Literature reference | EU directive 98/83
Food Additives and Contaminants, 2002, Vol. 19, No. 8, 721-732 |
| Chemical Abstract Service | 7726-95-6 (bromine – Br) |
| Cross reference |
Method number MW4
| Method of analysis | Gas chromatography with liquid-liquid extraction |
| Category of method of analysis | ISO 6468:1996 |
| Parameter group | Chemical (organochlorine insecticides) |
| Parameter | a. Polychlorinated biphenyls (PCB’s)
b. Chlorobenzenes |
| Legislative value | 0,10 μg/l (EU-pesticides)
0,50 μg/l (EU-pesticides – total) |
| Monitoring | |
| Literature reference | EU directive 98/83/EC |
| Chemical Abstract Service | a. 1336-36-3
b. 108-90-7 |
| Cross reference |
Method number MW5
| Method of analysis | Gas chromatography after solid phase extraction and derivatization |
| Category of method of analysis | ISO 15913:2000 |
| Parameter group | Chemical (phenoxyalkanoic herbicides) |
| Parameter | a. bentazones
b. hydroxybenzonitrils |
| Legislative value | 0,10 μg/l (EU-pesticides)
0,50 μg/l (EU-pesticides – total) |
| Monitoring | |
| Literature reference | EU directive 98/83/EC |
| Chemical Abstract Service | a. 25057-89-0b. |
| Cross reference |
Method number MW6
| Method of analysis | Gas chromatography / mass spectrometry – GC/MS (with liquid phase microextraction – LPME) |
| Category of method of analysis | ISO 18856:2004 |
| Parameter group | Chemical (plasticizer) |
| Parameter | Phthalate esters |
| Legislative value | |
| Monitoring | |
| Literature reference | Journal of Chromatography A, 30 May 2003, 999 (1-2), 145-153 (Psillakis, 2003) |
| Chemical Abstract Service | |
| Cross reference |
Method number MW7
| Method of analysis | Gas chromatography mass spectrometry (GS/MS) with solid phase microextraction |
| Category of method of analysis | |
| Parameter group | Chemical (antioxidant in packaging plastics) |
| Parameter | Butylated hydroxytoluene (BHT) |
| Legislative value | |
| Monitoring | |
| Literature reference | Journal of Chromatography A, july 2002, 963 (1-2), 179-183 (Tombesi, 2002) |
| Chemical Abstract Service | 128-37-0 |
| Cross reference |
Method number MW8
| Method of analysis | Ion chromatography (IC) with colorimetric detection |
| Category of method of analysis | |
| Parameter group | Chemical |
| Parameter | Chromium |
| Legislative value | 50 μg/l (EU) |
| Monitoring | |
| Literature reference | EU directive 2003/40/EC
Food Additives and Contaminants, 2002, Vol. 19, No. 8, 721-732 |
| Chemical Abstract Service | 7440-47-3 (Chromium VI - Cr) |
| Cross reference |
Method number MW9
| Method of analysis | Inductively coupled plasma mass spectrometry (ICPMS) |
| Category of method of analysis | ISO 17294-2:2003 |
| Parameter group | Chemical (trace elements) |
| Parameter | a. Arsenic
b. Boron c. Cadmium d. Copper e. Nickel f. Lead g. Selenium |
| Legislative value | a. 10 μg/l (EU 2003/40)
b. 1,0 mg/l (EU 98/83) c. 5,0 μ/l (EU 2003/40) d. 1,0 mg/l (EU 2003/40) e. 20 μg/l (EU 2003/40) f. 10 μg/l (EU 2003/40) g. 10 μg/l (EU 2003/40) |
| Monitoring | |
| Literature reference | EU directive 98/83/EC
EU directive 2003/40/EC Food Additives and Contaminants, 2002, Vol. 19, No. 8, 721-732 |
| Chemical Abstract Service | a. 7440-38-2 (Arsenic - As)
b. 7440-42-8 (Boron - B) c. 7440-43-9 (Cadmium – Cd) d. 7440-50-8 (Copper – Cu) e. 7440-02-0 (Nickel – Ni) f. 7439-92-1 (Lead – Pb) g. 7782-49-2 (Selenium – Se) |
| Cross reference |
Method number MW10
| Method of analysis | Capillary zone electrophoresis |
| Category of method of analysis | |
| Parameter group | Chemical (trace elements) |
| Parameter | a. Barium (II)
b. Strontium (II) |
| Legislative value | a. 700 μg/l (WHO, 1998)
a. 1,0 mg/l (EU 2003/40) b. No guideline |
| Monitoring | |
| Literature reference | Journal of Chromatography A. 1997, 767 (1-2) 303 – 310 (Macka – Miroslav, 1997)
EU directive 2003/40/EC |
| Chemical Abstract Service | a. 7440-39-3
b. 7440-24-6 |
| Cross reference |
Method number MW11
| Method of analysis | Isotope ratio analysis |
| Category of method of analysis | |
| Parameter group | Chemical |
| Parameter | H-2 (deutrium), O-18, H-3 (tritium), C-12, C-14, S-34 |
| Legislative value | |
| Monitoring | |
| Literature reference | Isotopes in environmental and health studies. 1996, 32 (4), 387-403 (Von Storch, 1996) |
| Chemical Abstract Service | |
| Cross Reference |
Method number MW12
| Method of analysis | Isotope ratio mass spectrometry |
| Category of method of analysis | |
| Parameter group | Chemical |
| Parameter | C-13 and C-14 |
| Legislative value | |
| Monitoring | |
| Literature reference | Food Chemistry 92 (2005) 507-514 (R. Redondo, 2005) |
| Chemical Abstract Service | |
| Cross reference |
Method number MW13
| Method of analysis | Gas chromatography LC/MS/MS |
| Category of method of analysis | |
| Parameter group | Chemical |
| Parameter | acrylamide |
| Legislative value | 0,10 µg/l (EU); 0,5 µg/l (WHO) |
| Monitoring | |
| Literature reference | http://www.opsi.gov.uk/legislation/wales/wsi2003/20033042e.htm
EU directive 98/83/EC |
| Chemical Abstract Service | 79-06-1 |
| Cross reference |
Method number MW14
| Method of analysis | Fluoride-ion selective electrode |
| Category of method of analysis | |
| Parameter group | Chemical |
| Parameter | Fluoride |
| Legislative value | 5,0 mg/l (EU) |
| Monitoring | |
| Literature reference | EU directive 2003/40/EC |
| Chemical Abstract Service | 16984-48-8 |
| Cross reference |
Method number MW15
| Method of analysis | Ion selective electrode (ISE) |
| Category of method of analysis | |
| Parameter group | Chemical |
| Parameter | a. Nitrate
b. Nitrite |
| Legislative value | a. 50 mg/l (EU)
b. 0,1 mg.l (EU) |
| Monitoring | |
| Literature reference | EU directive 2003/40/EC |
| Chemical Abstract Service | 7727-37-9 (N) |
| Cross reference |
